A rapid electrophoretic method for the determination of the isoenzymes of serum lactate dehydrogenase.
نویسندگان
چکیده
A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore Ill strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.
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عنوان ژورنال:
- Clinical chemistry
دوره 12 5 شماره
صفحات -
تاریخ انتشار 1966